isolation and identification of bioactive compound producing rhodococcus spp. isolated from soil samples
Authors
abstract
the genus rhodococcus is gram positive bacterium which was classified into the family of nocardiaceae. the organism can be isolates from different sources, with capability to degrade diverse types of pollutant. nowadays, scientists reported a few secondary metabolites which are produced by rhodococci. hence, the present study tried to isolate rhodococci from different soil samples with the capability to produce antimicrobial metabolites. for this purpose 360 soil samples were collected from different types of soil. then the isolates were identified and characterized using api coryne kit. afterward antibacterial activity of the suspected bacteria was evaluated using well diffusion agar method against test bacteria. furthermore, the extracted metabolites were analysed using gc-ms. the results obtained from this study indicated that 4 out of 9 isolates were able to produce antimicrobial metabolites. the most effective isolates were r87 strain with the maximum inhibition zone of 35 mm and the least effective were r85 strain with zone of inhibition 20 mm against e. coli. the gc-mass chromatogram showed different special peaks and the identified compounds were cyclopenthasiloxane, azulene, 2-7 octadiene, docosane, cyclohexasiloxane, bis (3,5,5 trimethyhexyl, tetraedacane – 2 methyl, cycloheptasiloxane and heptadecanol. in general, isolation of native bacteria from environmental samples with antimicrobial property is an interesting aspect which could help us to ignore antibiotic resistant bacteria from our environment.
similar resources
Isolation and identification of bioactive compound producing Rhodococcus spp. isolated from soil samples
The genus Rhodococcus is gram positive bacterium which was classified into the family of Nocardiaceae. The organism can be isolates from different sources, with capability to degrade diverse types of pollutant. Nowadays, scientists reported a few secondary metabolites which are produced by Rhodococci. Hence, the present study tried to isolate Rhodococci from different soil samples with the cap...
full textAntimicrobial and Antioxidant Activity of Carotenoid Pigment Produced by Native Rhodococcus spp. Isolated From Soil
In recent years, scientists and researchers from all over the world pay much attention to carotenoid pigments as a substitute for antibiotics and synthetic chemical compounds with antioxidant property and the plants, various microorganisms including bacteria and algae are also capable of producing carotenoid pigments. In this research, the production of antimicrobial and antioxidant carotenoid ...
full textEvaluation of Heavy Metals Resistance in Biofilm Cells of Native Rhodococcus spp. Isolated from Soil
Background & Aims of the Study: Rhodococci according to possess large genome, their active metabolism and survival under extreme conditions, are highly regarded for biodegradation and bioremediation of different pollutants especially heavy metals in the environment. Biofilms are more resistant to heavy metals than free-swimming organisms. The aim of this study was biofilm forma...
full textisolation and identification of nocardia spp. using phenotypic methods from soil samples of north khorasan province
introduction: nocardia is saprophytic soil bacterium of the actinomycetes families. nocardia has high diversity of species and due to species diversity of nocardia, phenotypic tests are essential for early identification of nocardia species. methods: 155 soil samples from different regions of north khorasan province in iran were collected, including: urban and rural areas. isolation of nocardia...
full textMy Resources
Save resource for easier access later
Journal title:
international journal of molecular and clinical microbiologyجلد ۵، شماره ۱، صفحات ۴۶۳-۴۶۸
Hosted on Doprax cloud platform doprax.com
copyright © 2015-2023